质谱分析
质谱中心
The Center for 质谱分析 (CMS) facility at The Merkert Chemistry Center maintains a wide range of state-of-the-art mass spectrometers and chromatographic systems, providing the full suite of ionization and separation options that are capable of dealing with all sample types and a wide array of client demands. 可用的电离方法包括ESI, 飞镖, 和谱技术, all of which are capable of operating in both the positive and negative ion modes.
样品一般由工厂工作人员操作, 结果以电子数据文件的形式提供. 在特殊情况下, an individual researcher who makes frequent use of a particular spectrometer may receive training and become authorized to run samples personally. Such authorized users then often run samples for other members of their own research group to reduce the pressure on the facility staff.
If you are an external user seeking to use Merkert CMS facilities, please contact Mr. Marek多明来讨论你的项目.
Please note that all work performed in BC core facilities and recharge centers should always be appropriately acknowledged. If you are publishing or presenting data acquired in BC core facilities and recharge centers, please include the following statement in the Acknowledgement section of your manuscript/poster/presentation, "The authors would like to thank the 电子游戏软件
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完整设施描述
1. 建议表达CMS
表达CMS是一个单四极质谱计, 配备ESI和APCI探针, 以最小的努力切换.
The instrument has a mass range of m/z 10 to m/z 1,200, and a resolution of 0.5-0.7m /z，单位为1000m /z，在整个采集范围内.
In Full scan mode the CMS has sensitivity for Reserpine of 100pg and in SIM mode of 10pg, 5μl进样量为100μl/min.
2. JEOL AccuTOF 飞镖
JEOL AccuTOF, configured with a new Direct Analysis in Real Time (飞镖) ion source, 代表了质谱学的重大突破. 这是第一台可以分析固体的质谱仪, liquids and gaseous samples at atmospheric pressure and ground potential by simply placing the sample between the 飞镖 and the mass spectrometer. 不需要溶剂或样品制备. 系统设计结果高吞吐量，无结转, 即使是纯聚乙二醇(PEG). The 飞镖 can even analyze samples directly from a variety of surfaces such as concrete, 货币, 食物, 药片, 服装, TLC板. The AccuTOF 飞镖 combination achieves high specificity through high resolution. Exact mass measurements together with precise isotopic abundances can rapidly identify unknown compounds by their elemental compositions.
The 飞镖 ion source is based on the reactions of excited-state species with reagent molecules and polar or non-polar analytes. It is free of the limitations that are common to other atmospheric pressure ion sources, which require direct exposure of gaseous or vaporized liquid samples to elevated temperatures and electrical potentials, 紫外线照射, 激光辐射, 或者是高速气流. 飞镖不会以任何方式改变样品, nor does it expose the user to a potentially harmful substance or dangerous situation.
3. Agilent 6220飞行时间LC/MS
The Agilent 6220 TOF LC/MS delivers exceptional analytical performance, 包括2 ppm质量精度, 是蛋白质和代谢物分析的理想选择. The powerful combination of resolving power and high mass accuracy allows identification of impurities in complex matrices faster and with greater confidence than with other methods.
耦合到质谱仪, a 1200 series rapid resolution LC system offers a unique combination of flexibility, 分辨率和速度. Benefits include the ability to run both rapid resolution and conventional HPLC methods on the same chromatography system, 哪一种既支持标准柱又支持窄孔柱. 快速解决, 反过来, 提供比传统HPLC高60%的分辨率, 分析时间大约快20倍, 与标准HPLC相比.
4. Agilent 6460三重四轴
The 6460 Triple Quad MS is a liquid chromatograph triple 四极 mass spectrometer that performs MS/MS using three sets of parallel rods (in this case, 四极, 六极, 四极). The first 四极 separates ions into precursor ions that are fragmented in the 六极 into product ions, 它们被第二个四极杆隔开. 经常, two or more precursor ions and their product ions are monitored in sequence in MRM (multiple reaction monitoring) mode. 通过使用Dynamic MRM，您可以监控多达4000个MRM转换.
布鲁克Autoflex Max
布鲁克Autoflex Max MALDI-TOF mass spectrometer can analyze ions over a wide m/z range, 能够分析广谱的分子, 从小分子开始, 比如脂质和多肽, 直到聚合物和大于100 kDa的完整蛋白质. 他们的PAN全景对焦和新的10位数字化仪, 保持峰值分辨率, and enhances dynamic range for a more complete view of low abundance sample components. 在MALDI MS成像应用, this expansion of dynamic range can be invaluable for complex samples such as tissue, 但也有利于所有的应用, 包括直接TLC-MALDI, 聚合物表征.
质谱仪培训申请程序:
目前, after completing the required training users will have access to the JEOL AccuTOF 飞镖 and Bruker MALDI systems. 培训请求可以并且应该通过实验室发起, in order to arrange a suitable date and time for training to be carried out. This is followed by a period of supervised instrument time before any access is granted for off hours works.
马立克·多明，m.mSc FRSC CChem CSci
质谱中心主任
Mr. Marek多明 joined the Chemistry Department at 电子游戏软件 in August 2005. 他是土生土长的英国人. Domin worked at the School of Pharmacy in London for ten years as part a large mass spectrometry group. 他获得了硕士学位。.Sc. 伯克贝克学院化学电子游戏正规平台学位, 他在哪里使用毛细管电泳, as an alternative to gas chromatography – mass spectrometry for the detection of modified DNA bases by hydroxyl radicals. 在电子游戏正规平台生学习期间, he carried out research into the characterization of micro-organisms using MALDI-TOF. Mr. 多明被选为皇家化学学会会员, 编辑委员会, 《电子游戏正规平台》系列丛书, 还有胡安·加西亚·雷耶斯教授和弗兰克·索伯特教授. His research interests include the application of mass spectrometry as a routine tool for chemistry/chemical biology; exploring new research applications for mass spectrometry; reaction monitoring by mass spectrometry; and the use of open air ionization methods for the analysis of pharmaceutical, 生物, 化学分析.
质谱中心
马立克·多明，m.m.Sc.
导演
毕顿街245号
Chestnut Hill MA 02467-3808
工作:617.552.3077
手机:617-895-7739
电子邮件: domin@repjcclothing.com